Publication dateMar 9, 2022
Confidentiality public Public
Typing name : TASK.gws_ubiome.Qiime2QualityCheck Brick : gws_ubiome Performs a sequencing quality check analysis with Qiime2
Qiime2QualityCheck class.
This task examines the quality of the metabarcoding sequences using the function demux summarize
from Qiime2. Both paired-end and single-end sequences can be used, but sequences have to be demultiplexed first. It generates interactive positional quality plots based on randomly selected sequences, and the quality plots present the average positional qualities across all of the sequences selected. Default parameter is used, i.e. 10,000 random sequences are selected to generate quality plots.
More information here https://docs.qiime2.org/2022.8/plugins/available/demux/summarize/
[Mandatory]:
- fastq_folder must contains all fastq files (paired or not).
- metadata file must follow a specific nomenclature when columns are tab separated. The gws_ubiome task 'Qiime2 metadata table maker' automatically generates a ready-to-use
metadata file when given a fastq folder as input. You can also upload your own metadata file.
For paired-end files :
sample-id forward-absolute-filepath reverse-absolute-filepath
sample-1 sample0_R1.fastq.gz sample1_R2.fastq.gz
sample-2 sample2_R1.fastq.gz sample2_R2.fastq.gz
sample-3 sample3_R1.fastq.gz sample3_R2.fastq.gz
For single-end files :
sample-id absolute-filepath
sample-1 sample0.fastq.gz
sample-2 sample2.fastq.gz
sample-3 sample3.fastq.gz
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Input
Fastq folder
Folder containing fastq files
A metadata file
A metadata file with at least sequencing file names
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Output
Resource set
A set of resources
settings
Configuration
Type of sequencing. Defaults to paired-end
Type : string
Allowed values : paired-end
single-end
Default value : paired-end
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