🔍 Introduction
This task wraps NCBI’s web BLAST service to run per-sequence remote BLAST directly from Constellab. It automatically splits your FASTA into single-sequence queries, submits them to NCBI, waits politely between requests, and collects results as clean .tsv tables one per query.
Use it when you want up-to-date results against NCBI’s online databases without maintaining local BLAST databases.
🧰 Prerequisites
- Access to Constellab and a valid Digital Lab environment
- Installed bricks:
gws_omix ≥ 0.11.11
- A FASTA file (nucleotide or protein)
🧪 Workflow: Step by Step
- Add the task: Choose NCBI web BLAST in Constellab.
- Provide input: Link your
input_fasta.
- Configure parameters:
Choose
blast_program consistent with your sequence type:
blastn → sequence_type=nuclblastp → sequence_type=protblastx → sequence_type=nucl (nucl query vs protein DB)
tblastn → sequence_type=prot (prot query vs translated nucl DB)Select sequence_type: "nucl" (nucleotide) or "prot" (protein).
Set evalue (e.g., 1e-5 for stricter matches).
Set max_target_seqs (e.g., 5–50 depending on breadth vs precision).
- Run the task
📤 Output
NCBI web BLAST task produces a blast_results ResourceSet containing one .tsv file per query sequence. Each table includes:
