Publication dateMar 9, 2022
Confidentiality public Public
Typing name : TASK.gws_omix.Trimmomatic Brick : gws_omix Eliminate adapters and low-quality reads
Trimmomatic is a software tool that trims and filters high-throughput sequencing data.
It removes adapter sequences, low-quality reads, and other unwanted artifacts.
Compatible with both single-end and paired-end data.
Outputs are named so that, for paired-end data, the final files become:
trimmed{Forward_separator}.fastq.gz
trimmed{Reverse_separator}.fastq.gz
Then HISAT2 or any other aligner can detect them using the same Forward/Reverse separators.
login
Input
FASTQ Folder
Folder containing raw FASTQ reads
logout
Output
Trimmed Reads
Folder containing trimmed FASTQ files
settings
Configuration
Number of threads
Type : intDefault value : 2Minimum read length to keep
Type : intDefault value : 35Choose Single-end or Paired-end
Type : stringAllowed values : Paired-end Single-end Forward read identifier (for Paired-end)
Type : stringAllowed values : R1 1 r1 Reverse read identifier (for Paired-end)
Type : stringAllowed values : R2 2 r2 5_prime_hard_trimming_read_size
Optional
Bases to remove from 5' end
Type : intTechnical bricks to reuse or customize
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