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Mar 9, 2022

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Fastp

TASK
52 times
100 %
8 minutes, 4 seconds
Typing name :  TASK.gws_omix.Fastp Brick :  gws_omix

Adapters removal with fastp

This task trims adapters and low-quality bases using fastp for FASTQ datasets defined by a metadata TSV. Provide a fastq_folder (base path for relative file references) and a metadata file that is either single-end (contains absolute-filepath) or paired-end (contains both forward-absolute-filepath and reverse-absolute-filepath). Key options: - threads - Forward_separator/Reverse_separator (suffixes like 1/2), - five_prime_hard_trimming_read_size (bases trimmed from the 5′ end applied to both mates in PE).

The task auto-resolves relative paths against fastq_folder, executes fastp, and returns a FastqFolder pointing to the result directory.

Input

FASTQ folder
Folder containing raw FASTQ(.gz); relative paths in metadata start from here
Metadata (TSV)
Table with absolute-filepath (SE) or forward- & reverse-absolute-filepath (PE)

Output

Trimmed FASTQ
Folder with *_trimmed*.fastq.gz files

Configuration

threads

Optional

Type : intDefault value : 4

Forward_separator

Optional

Type : string

Reverse_separator

Optional

Type : string

five_prime_hard_trimming_read_size

Optional

Type : int
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