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Introduction Version

DESeq2 pairwise differential analysis

TASK
Typing name :  TASK.gws_omix.DESeq2DifferentialAnalysis Brick :  gws_omix

Compute differential analysis using DESeq2 R package (pairwise comparison)

DESeq2DifferentialAnalysis class.

Input

Salmon_merged_counts
Salmon merged raw count files
Metadata_file
Metadata file describing samples (see https://hub.gencovery.com/bricks/gws_omix/latest/doc/use-cases/undefined )

Output

Deseq2 output files
DEseq2 output tables containing fold-change and statistics
Deseq2 output folder
DEseq2 output folder containing all output files
Deseq2 summary file
DEseq2 output file which summarise under the threshold genes differentially expressed

Configuration

metadata_column

Optional

Column on which the differential analysis will be performed

Type : string

output_file_names

Optional

Choose the output file names (e.g. output_file --> output-file.XXX.txt, ...)

Type : stringDefault value : DESeq2

summary_threshold

Optional

Choose the threshold used to filter adjusted p-value to generate summary file

Type : floatDefault value : 0.05