DESeq2 pairwise differential analysis
Compute differential analysis using DESeq2 R package (pairwise comparison)
DESeq2DifferentialAnalysis class.
Input
Salmon_merged_counts
Salmon merged raw count files
Metadata_file
Metadata file describing samples (see https://hub.gencovery.com/bricks/gws_omix/latest/doc/use-cases/undefined )
Output
Deseq2 output files
DEseq2 output tables containing fold-change and statistics
Deseq2 output folder
DEseq2 output folder containing all output files
Deseq2 summary file
DEseq2 output file which summarise under the threshold genes differentially expressed
Configuration
metadata_column
Column on which the differential analysis will be performed
string
output_file_names
Choose the output file names (e.g. output_file --> output-file.XXX.txt, ...)
string
DESeq2
summary_threshold
Choose the threshold used to filter adjusted p-value to generate summary file
float
0.05