PCR Method

Technical name
pu_pcr_method
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Publication date

Oct 3, 2025

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Description

Controlled vocabulary of PCR methods used in molecular biology and diagnostics.

Predefined tag values

ValueShort descriptionAdditional infoStatus
Digital PCR
Partitioned PCR for absolute quantification
category :  digital application :  Rare mutation detection, absolute quantification
Active
Multiplex PCR
Simultaneous amplification of multiple targets
category :  specialized application :  Pathogen panels, genetic testing
Active
RT-PCR
Reverse transcription PCR
category :  qualitative application :  RNA to cDNA amplification
Active
Nested PCR
Two-step PCR for higher specificity
category :  specialized application :  Detection of low abundance templates
Active
qPCR
Quantitative real-time PCR
category :  quantitative application :  Gene expression analysis, viral load quantification
Active
Isothermal PCR (LAMP, RPA)
Amplification at constant temperature
category :  isothermal application :  Point-of-care diagnostics
Active
Conventional PCR
Standard PCR for DNA amplification
category :  qualitative application :  General DNA amplification
Active
RT-qPCR
Quantitative reverse transcription PCR
category :  quantitative application :  Quantification of RNA transcripts
Active
Hot-start PCR
Enzyme-activated PCR to improve specificity
category :  specialized application :  Minimizing non-specific amplification
Active

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